The story of the game shared similarities to the story of the Assassin's Creed: Brotherhood, aside from a few exceptions:
Lucrezia and Caterina were located in Florence, as opposed to the Castel Sant'Angelo in Rome.
Ezio was required to kill Lucrezia Borgia in order to free Caterina Sforza, instead of capturing her.
Claudio, a member of the Thieves Guild of Rome, was captured by the Followers of Romulus.
The leader of the Followers was Romulus himself.
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